Nature Methods 3, 605 - 607 (2006)
Published online: 21 July 2006; | doi:10.1038/nmeth900
Removal of a time barrier for high-resolution multidimensional NMR spectroscopyVictor Jaravine1, Ilgis Ibraghimov2 & Vladislav Yu Orekhov11
The Swedish NMR Centre at Gothenburg University, Box 465, 40530 Gothenburg, Sweden. 2
Saarbrücken University, Mathematical Department, Saarbrücken, D-66041, Germany.
Correspondence should be addressed to Vladislav Yu Orekhov orov@nmr.gu.se We introduce the recursive multidimensional decomposition (R-MDD) method to speed recording of high-resolution NMR spectra. The measurement time is logarithmically dependent on the sizes of indirect spectral dimensions. R-MDD has the sensitivity and resolution advantages of optimized nonuniform acquisition schemes and is applicable to all types of biomolecular spectra. We demonstrated it for triple resonance experiments on three globular proteins (ubiquitin, azurin and the barstar-barnase complex) of 8–22 kDa.
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