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| In This Issue | Top |
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| Editorial | Top |
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A picture worth a thousand words (of explanation) p237 doi:10.1038/nmeth0406-237 Full text | PDF
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| Research Highlights | Top |
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Putting long-range mapping in reach p239 Michael Eisenstein doi:10.1038/nmeth0406-239 Abstract | Full text | PDF
(128K)
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Bioluminescent quantum dots pp240 - 241 Daniel Evanko doi:10.1038/nmeth0406-240a Abstract | Full text | PDF
(165K)
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Harnessing a tiny hairy beast pp240 - 241 Irene Kaganman doi:10.1038/nmeth0406-240b Abstract | Full text | PDF
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News in Brief p241 doi:10.1038/nmeth0406-241 Full text | PDF
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Improving evolution p242 Nicole Rusk doi:10.1038/nmeth0406-242 Abstract | Full text | PDF
(70K)
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Hands-off protein crystallography p244 Allison Doerr doi:10.1038/nmeth0406-244a Abstract | Full text | PDF
(74K)
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Coke and chips—a winning combination? p244 Michael Eisenstein doi:10.1038/nmeth0406-244b Abstract | Full text | PDF
(74K)
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| News and Views | Top |
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Chimeric streptavidins with reduced valencies pp247 - 248 Guillaume Lemercier
& Kai Johnsson doi:10.1038/nmeth0406-247 Abstract | Full text | PDF
(620K)
See also: Article by Howarth et al. |
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Myeloid progenitors on demand pp248 - 249 Dan A Liebermann
& Barbara Hoffman doi:10.1038/nmeth0406-248 Abstract | Full text | PDF
(288K)
See also: Article by Wang et al. |
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| Review | Top |
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Analyzing phosphoinositides and their interacting proteins pp251 - 258 Tor Erik Rusten
& Harald Stenmark Published online: 22 March 2006 | doi:10.1038/nmeth867 Abstract | Full text | PDF
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| Brief Communications | Top |
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Imaging specific cell-surface proteolytic activity in single living cells pp259 - 261 John P Hobson, Shihui Liu, Birgitte Rønø, Stephen H Leppla
& Thomas H Bugge Published online: 22 March 2006 | doi:10.1038/nmeth862 Abstract | Full text | PDF
(456K)
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Efficient incorporation of unnatural amino acids into proteins in Escherichia coli pp263 - 265 Youngha Ryu
& Peter G Schultz Published online: 22 March 2006 | doi:10.1038/nmeth864 Abstract | Full text | PDF
(168K)
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| Articles | Top |
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A monovalent streptavidin with a single femtomolar biotin binding site pp267 - 273 Mark Howarth, Daniel J-F Chinnapen, Kimberly Gerrow, Pieter C Dorrestein, Melanie R Grandy, Neil L Kelleher, Alaa El-Husseini
& Alice Y Ting Published online: 22 March 2006 | doi:10.1038/nmeth861 Abstract | Full text | PDF
(374K)
| Supplementary Information See also: News and Views by Lemercier & Johnsson |
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Slowed diffusion in tumors revealed by microfiberoptic epifluorescence photobleaching pp275 - 280 Jay R Thiagarajah, Jung Kyung Kim, Mazin Magzoub
& A S Verkman Published online: 22 March 2006 | doi:10.1038/nmeth863 Abstract | Full text | PDF
(389K)
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Genetically encoded fluorescent indicator for intracellular hydrogen peroxide pp281 - 286 Vsevolod V Belousov, Arkady F Fradkov, Konstantin A Lukyanov, Dmitry B Staroverov, Konstantin S Shakhbazov, Alexey V Terskikh
& Sergey Lukyanov Published online: 22 March 2006 | doi:10.1038/nmeth866 Abstract | Full text | PDF
(342K)
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Quantitative production of macrophages or neutrophils ex vivo using conditional Hoxb8 pp287 - 293 Gang G Wang, Katherine R Calvo, Martina P Pasillas, David B Sykes, Hans Häcker
& Mark P Kamps Published online: 22 March 2006 | doi:10.1038/nmeth865 Abstract | Full text | PDF
(519K)
| Supplementary Information See also: News and Views by Liebermann & Hoffman |
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Luminescent imaging of -galactosidase activity in living subjects using sequential reporter-enzyme luminescence pp295 - 301 Thomas S Wehrman, Georges von Degenfeld, Peter O Krutzik, Garry P Nolan
& Helen M Blau Published online: 22 March 2006 | doi:10.1038/nmeth868 Abstract | Full text | PDF
(468K)
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| Protocol | Top |
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Optimization of membrane protein overexpression and purification using GFP fusions pp303 - 313 David Drew, Mirjam Lerch, Edmund Kunji, Dirk-Jan Slotboom
& Jan-Willem de Gier doi:10.1038/nmeth0406-303 Full text | PDF
(366K)
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| Technology Feature | Top |
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Understanding how proteins accessorize pp315 - 323 Caitlin Smith doi:10.1038/nmeth0406-315 Abstract | Full text | PDF
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| Classic Protocol | Top |
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A look back: putting differences aside p324 Michael Eisenstein doi:10.1038/nmeth0406-324 Full text | PDF
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Differential display polymerase chain reaction pp325 - 326 doi:10.1038/nmeth0406-325 Full text | PDF
(56K)
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| Erratum | Top |
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Rapid analysis of T-cell selection in vivo using T cell–receptor retrogenic mice p327 Jeff Holst, Kate M Vignali, Amanda R Burton
& Dario A A Vignali doi:10.1038/nmeth0406-327 Full text | PDF
(43K)
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| Application Notes | Top |
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MicroRNA expression profiling on arrays enhanced with locked nucleic acids Peter Roberts doi:10.1038/nmeth869 Abstract | Full text | PDF
(276K)
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Fast and reliable MALDI-TOF MS–based microorganism identification Thomas Maier, Stefan Klepel, Uwe Renner
& Markus Kostrzewa Published online: 22 March 2006 | doi:10.1038/nmeth870 Abstract | Full text | PDF
(248K)
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