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Nature Methods
ISSUE
April 2006, Volume 3 No 4
In This Issue
Editorial
Research Highlights
News and Views
Review
Brief Communications
Articles
Protocol
Technology Feature
Classic Protocol
Erratum
Application Notes
About the cover
In This Issue Top
PDF
Editorial Top
A picture worth a thousand words (of explanation) p237
doi:10.1038/nmeth0406-237
Full text | PDF (46K)
Research Highlights Top
Putting long-range mapping in reach p239
Michael Eisenstein
doi:10.1038/nmeth0406-239
Abstract | Full text | PDF (128K)
Bioluminescent quantum dots pp240 - 241
Daniel Evanko
doi:10.1038/nmeth0406-240a
Abstract | Full text | PDF (165K)
ADVERTISEMENT
Harnessing a tiny hairy beast pp240 - 241
Irene Kaganman
doi:10.1038/nmeth0406-240b
Abstract | Full text | PDF (165K)
News in Brief p241
doi:10.1038/nmeth0406-241
Full text | PDF (78K)
Improving evolution p242
Nicole Rusk
doi:10.1038/nmeth0406-242
Abstract | Full text | PDF (70K)
Hands-off protein crystallography p244
Allison Doerr
doi:10.1038/nmeth0406-244a
Abstract | Full text | PDF (74K)
Coke and chips—a winning combination? p244
Michael Eisenstein
doi:10.1038/nmeth0406-244b
Abstract | Full text | PDF (74K)
News and Views Top
Chimeric streptavidins with reduced valencies pp247 - 248
Guillaume Lemercier & Kai Johnsson
doi:10.1038/nmeth0406-247
Abstract | Full text | PDF (620K)
See also: Article by Howarth et al.
Myeloid progenitors on demand pp248 - 249
Dan A Liebermann & Barbara Hoffman
doi:10.1038/nmeth0406-248
Abstract | Full text | PDF (288K)
See also: Article by Wang et al.
Review Top
Analyzing phosphoinositides and their interacting proteins pp251 - 258
Tor Erik Rusten & Harald Stenmark
Published online: 22 March 2006 | doi:10.1038/nmeth867
Abstract | Full text | PDF (500K)
Brief Communications Top
Imaging specific cell-surface proteolytic activity in single living cells pp259 - 261
John P Hobson, Shihui Liu, Birgitte Rønø, Stephen H Leppla & Thomas H Bugge
Published online: 22 March 2006 | doi:10.1038/nmeth862
Abstract | Full text | PDF (456K)  | Supplementary Information
Efficient incorporation of unnatural amino acids into proteins in Escherichia coli pp263 - 265
Youngha Ryu & Peter G Schultz
Published online: 22 March 2006 | doi:10.1038/nmeth864
Abstract | Full text | PDF (168K)  | Supplementary Information
Articles Top
A monovalent streptavidin with a single femtomolar biotin binding site pp267 - 273
Mark Howarth, Daniel J-F Chinnapen, Kimberly Gerrow, Pieter C Dorrestein, Melanie R Grandy, Neil L Kelleher, Alaa El-Husseini & Alice Y Ting
Published online: 22 March 2006 | doi:10.1038/nmeth861
Abstract | Full text | PDF (374K)  | Supplementary Information
See also: News and Views by Lemercier & Johnsson
Slowed diffusion in tumors revealed by microfiberoptic epifluorescence photobleaching pp275 - 280
Jay R Thiagarajah, Jung Kyung Kim, Mazin Magzoub & A S Verkman
Published online: 22 March 2006 | doi:10.1038/nmeth863
Abstract | Full text | PDF (389K)  | Supplementary Information
Genetically encoded fluorescent indicator for intracellular hydrogen peroxide pp281 - 286
Vsevolod V Belousov, Arkady F Fradkov, Konstantin A Lukyanov, Dmitry B Staroverov, Konstantin S Shakhbazov, Alexey V Terskikh & Sergey Lukyanov
Published online: 22 March 2006 | doi:10.1038/nmeth866
Abstract | Full text | PDF (342K)  | Supplementary Information
Quantitative production of macrophages or neutrophils ex vivo using conditional Hoxb8 pp287 - 293
Gang G Wang, Katherine R Calvo, Martina P Pasillas, David B Sykes, Hans Häcker & Mark P Kamps
Published online: 22 March 2006 | doi:10.1038/nmeth865
Abstract | Full text | PDF (519K)  | Supplementary Information
See also: News and Views by Liebermann & Hoffman
Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence pp295 - 301
Thomas S Wehrman, Georges von Degenfeld, Peter O Krutzik, Garry P Nolan & Helen M Blau
Published online: 22 March 2006 | doi:10.1038/nmeth868
Abstract | Full text | PDF (468K)  | Supplementary Information
Protocol Top
Optimization of membrane protein overexpression and purification using GFP fusions pp303 - 313
David Drew, Mirjam Lerch, Edmund Kunji, Dirk-Jan Slotboom & Jan-Willem de Gier
doi:10.1038/nmeth0406-303
Full text | PDF (366K)  | Supplementary Information
Technology Feature Top
Understanding how proteins accessorize pp315 - 323
Caitlin Smith
doi:10.1038/nmeth0406-315
Abstract | Full text | PDF (306K)
Classic Protocol Top
A look back: putting differences aside p324
Michael Eisenstein
doi:10.1038/nmeth0406-324
Full text | PDF (46K)
Differential display polymerase chain reaction pp325 - 326
doi:10.1038/nmeth0406-325
Full text | PDF (56K)
Erratum Top
Rapid analysis of T-cell selection in vivo using T cell–receptor retrogenic mice p327
Jeff Holst, Kate M Vignali, Amanda R Burton & Dario A A Vignali
doi:10.1038/nmeth0406-327
Full text | PDF (43K)
Application Notes Top
MicroRNA expression profiling on arrays enhanced with locked nucleic acids 
Peter Roberts
doi:10.1038/nmeth869
Abstract | Full text | PDF (276K)
Fast and reliable MALDI-TOF MS–based microorganism identification 
Thomas Maier, Stefan Klepel, Uwe Renner & Markus Kostrzewa
Published online: 22 March 2006 | doi:10.1038/nmeth870
Abstract | Full text | PDF (248K)
  Top

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ISSN: 1548-7091
EISSN: 1548-7105
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