Nature Methods 3, 199 - 204 (2006)
Published online: 17 February 2006; | doi:10.1038/nmeth854
3' UTR seed matches, but not overall identity, are associated with RNAi off-targetsAmanda Birmingham, Emily M Anderson, Angela Reynolds, Diane Ilsley-Tyree, Devin Leake, Yuriy Fedorov, Scott Baskerville, Elena Maksimova, Kathryn Robinson, Jon Karpilow, William S Marshall
& Anastasia Khvorova Supplementary Fig. 1 (pdf 40K)
Variations of Smith-Waterman scoring parameters fail to improve the ability to distinguish off-targets from untargeted genes. Supplementary Fig. 2 (pdf 52K)
Web site tool for identification of potential off-targets based on 3UTR-hexamer seed matches. Supplementary Fig. 3 (pdf 20K)
Seed region-off-targeting association is not due to 3' UTR length. Supplementary Table 1 (pdf 40K)
List of siRNA used in study. Supplementary Table 2 (pdf 16K)
Table of validated and predicted off-targets. Supplementary Table 3 (pdf 24K)
Custom S-W scoring parameters. Supplementary Note (doc 20K)
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