Nature Methods
- 3, 1001 - 1006 (2006)
Published online: 5 November 2006; | doi:10.1038/nmeth978
A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transferJohn R James, Marta I Oliveira, Alexandre M Carmo, Andrea Iaboni & Simon J Davis Supplementary Fig. 1 (pdf 288K)
Residual analysis of BRET data for all constructs used. Supplementary Table 1 (pdf 28K)
Numerical representation of goodness-of-fits. Supplementary Methods (pdf 60K) Supplementary Note (pdf 68K)
Why the dependence of transfer efficiency (BRETeff) on acceptor/donor ratio is systematically different for randomly interacting proteins versus oligomeric proteins, without recourse to theory.
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