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Article
Nature Methods - 3, 1001 - 1006 (2006)
Published online: 5 November 2006; | doi:10.1038/nmeth978

A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer

John R James, Marta I Oliveira, Alexandre M Carmo, Andrea Iaboni & Simon J Davis

Supplementary Fig. 1 (pdf 288K)
Residual analysis of BRET data for all constructs used.

Supplementary Table 1 (pdf 28K)
Numerical representation of goodness-of-fits.

Supplementary Methods (pdf 60K)

Supplementary Note (pdf 68K)
Why the dependence of transfer efficiency (BRETeff) on acceptor/donor ratio is systematically different for randomly interacting proteins versus oligomeric proteins, without recourse to theory.


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Nature Methods
ISSN: 1548-7091
EISSN: 1548-7105
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