Nature Methods
- 3, 1013 - 1019 (2006)
Published online: 22 October 2006; Corrected online: 6 October 2006 | doi:10.1038/nmeth968
An efficient tandem affinity purification procedure for interaction proteomics in mammalian cellsTilmann Bürckstümmer, Keiryn L Bennett, Adrijana Preradovic, Gregor Schütze, Oliver Hantschel, Giulio Superti-Furga & Angela Bauch Supplementary Fig. 1 (pdf 548K)
Detailed characterization of the AC- and the GS-TAP-tag. Supplementary Fig. 2 (pdf 2M)
GS-TAP-Ku70: Biochemical activity and specificity control. Supplementary Fig. 3 (pdf 2M)
The GS-TAP tag allows for a single-step purification. Supplementary Table 1 (doc 2M)
Lists of interactors associated with Ku70 and nonspecific interactors as identified my mass spectrometry analysis. Supplementary Methods (doc 48K)
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