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Article
Nature Methods - 3, 1007 - 1012 (2006)
Published online: 22 October 2006; | doi:10.1038/nmeth965

Two-chamber AFM: probing membrane proteins separating two aqueous compartments

Rui Pedro Gonçalves, Guillaume Agnus, Pierre Sens, Christine Houssin, Bernard Bartenlian & Simon Scheuring

Supplementary Fig. 1 (pdf 664K)
Schematic representation of the 2-chamber AFM setup.

Supplementary Fig. 2 (pdf 1M)
AFM characterization of the holey Si(001) support wafers.

Supplementary Video 1 (mov 468K)
Proton pumping of non-supported purple membranes. Sequence of green fluorescence images over a time-range of 500 seconds documenting fluorescence intensity variations in chambers.

Supplementary Note (pdf 52K)
Digital image treatment


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Nature Methods
ISSN: 1548-7091
EISSN: 1548-7105
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