Nature Methods
- 3, 1007 - 1012 (2006)
Published online: 22 October 2006; | doi:10.1038/nmeth965
Two-chamber AFM: probing membrane proteins separating two aqueous compartmentsRui Pedro Gonçalves, Guillaume Agnus, Pierre Sens, Christine Houssin, Bernard Bartenlian & Simon Scheuring Supplementary Fig. 1 (pdf 664K) Schematic representation of the 2-chamber AFM setup. Supplementary Fig. 2 (pdf 1M) AFM characterization of the holey Si(001) support wafers. Supplementary Video 1 (mov 468K) Proton pumping of non-supported purple membranes. Sequence of green fluorescence images over a time-range of 500 seconds documenting fluorescence intensity variations in chambers. Supplementary Note (pdf 52K) Digital image treatment
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