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Brief Communication
Nature Methods 3, 977–979 (1 December 2006) | doi:10.1038/nmeth979
A highly sensitive protein-protein interaction assay based on Gaussia luciferase
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Abstract
Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels.
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