 | Figure 2
Nature Methods
- 3, 1001 - 1006 (2006)
Published online: 5 November 2006; | doi:10.1038/nmeth978
A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transferJohn R James, Marta I Oliveira, Alexandre M Carmo, Andrea Iaboni & Simon J Davis | | | | Figure 2. Monomeric proteins expressed at the cell surface give substantial resonance energy transfer in conventional BRET experiments.
(a) Schematic of the architecture of proteins used in this study, approximately to scale. Scale bar, 10 nm. (b) BRETeff for the indicated proteins expressed as BRET pairs, normalized against the value obtained for sGFP-Luc. "BP" denotes single proteins coexpressed as both Luc and GFP fusions. Error bars represent mean  s.d., n = 8. (c) FACS analysis of CD86GFP-transfected HEK 293T cells after 24 h, stained with anti–CD86-PE (Serotec). (d) Confocal microscopy–based analysis of CD86GFP (left) and  2ARGFP (right) expression, demonstrating GFP localization to the cell surface.
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