 | Figure 3
Nature Methods
- 3, 981 - 983 (2006)
Published online: 29 October 2006; | doi:10.1038/nmeth972
Protein interaction screening by quantitative immunoprecipitation combined with knockdown (QUICK)Matthias Selbach & Matthias Mann | | | | Figure 3. QUICK screen for interaction partners of Cbl.
(a) The known Cbl interaction partners CD2-associated protein (CD2AP), Cbl-interacting protein of 85 kDa (CIN85) and Grb2 precipitated with the highest abundance ratios. Moreover, sorting nexin 18 (SNX18) showed a high abundance ratio and thus appears to be a newly identified Cbl interaction partner. Several other proteins also showed increased abundance ratios and therefore may be associated with Cbl in vivo (possibly indirectly). However, they were not outliers according to our statistical test. (b) No significant abundance ratios were found among 526 proteins identified in the whole cell lysate used for the immunoprecipitation, indicating that siRNA transfection did not appear to induce major changes in the proteome.
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