Nature Methods
- 3, 977 - 979 (2006)
Published online: 12 November 2006; | doi:10.1038/nmeth979
A highly sensitive protein-protein interaction assay based on Gaussia luciferaseIngrid Remy & Stephen W Michnick
Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, Québec, H3C 3J7, Canada.
Correspondence should be addressed to Stephen W Michnick stephen.michnick@umontreal.ca Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels. We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGF signaling.
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