Nature Methods
- 3, 903 - 907 (2006)
Published online: 23 October 2006; | doi:10.1038/nmeth951
Stacking the deck: double-tiled DNA microarraysSarah J Wheelan1, Francisco Martínez-Murillo1, Rafael A Irizarry2 & Jef D Boeke11
The High Throughput Biology Center and Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, 733 North Broadway, BRB Suite 331 Baltimore, Maryland 21205, USA. 2
Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, E3132, Baltimore, Maryland 21205, USA.
Correspondence should be addressed to Jef D Boeke jboeke@jhmi.edu or Rafael A Irizarry ririzarr@jhsph.edu Microarrays—high-throughput platforms for analyzing the gene expression and features of total genomic DNA, among other applications—are gaining in popularity as researchers discover ever more uses for their unbiased and broad feature sets. At present, microarray analyses are limited by the number of individual features that can be placed on each array. Here we describe a double-tiling method that significantly increases the number of sequences present on an array, and we show that successful transcriptional profiling is possible and straightforward with such arrays. With this method, we and others can save money and precious samples by using fewer arrays to cover a region, or can carry out investigations at significantly higher resolution without incurring prohibitive costs or increasing the amount of sample required for the experiment.
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