Nature Methods
- 3, 833 - 838 (2006)
Published online: 21 September 2006; | doi:10.1038/nmeth935
Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assaysMeghana M Kulkarni, Matthew Booker, Serena J Silver, Adam Friedman, Pengyu Hong, Norbert Perrimon & Bernard Mathey-Prevot Supplementary Fig. 1 (pdf 520K)
Microarray analysis of off-target effects. Supplementary Fig. 2 (pdf 92K)
Flow-chart and recommendations for assessing the specificity of dsRNAs in genome-wide-screens. Supplementary Table 1 (xls 96K)
GO functional categories specifically enriched in dsRNAs that scored as hits in 5 or more screens. Supplementary Table 2 (xls 60K)
Analyzing the dependence between gene functions and the number of predicted off-targets. Supplementary Table 3 (pdf 36K)
Amplicon ID for dsRNAs used in Figure 3. Supplementary Table 4 (pdf 48K)
Phenotype of multiple amplicons targeting seven genes in ERK activation assay. Supplementary Table 5 (pdf 52K)
Assay reproducibility and discovery rate for known components of the Wg, Hh and JAK/STAT pathways measured in 3 genome-wide RNAi screens. Supplementary Methods (pdf 64K)
| 
