Figure - Nature Methods

Figure 1

Nature Methods - 3, 777 - 779 (2006)
doi:10.1038/nmeth1006-777

Minimizing the risk of reporting false positives in large-scale RNAi screens

Christophe J Echeverri, Philip A Beachy, Buzz Baum, Michael Boutros, Frank Buchholz, Sumit K Chanda, Julian Downward, Jan Ellenberg, Andrew G Fraser, Nir Hacohen, William C Hahn, Aimee L Jackson, Amy Kiger, Peter S Linsley, Lawrence Lum, Yong Ma, Bernard Mathey-Prévôt, David E Root, David M Sabatini, Jussi Taipale, Norbert Perrimon & René Bernards

Figure 1. Appropriate experimental controls to minimize risks of misinterpretation of RNAi data due to off-target effects (OTEs).
siRNA-like molecules, vector-based shRNAs and long dsRNAs trigger detectable off-target effects in all major systems studied to date, from mammalian cells to D. melanogaster and C. elegans. Simple solutions are available to minimize the risk that an observed phenotype may arise from an off-target effect rather than the targeted gene's loss of function.

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