Nature Methods
- 3, 793 - 796 (2006)
Published online: 9 August 2006; | doi:10.1038/nmeth929
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)Michael J Rust1, 5, Mark Bates2, 5 & Xiaowei Zhuang1, 3, 41
Department of Physics, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA. 2
Division of Engineering and Applied Sciences, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA. 3
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA. 4
Howard Hughes Medical Institute, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA. 5
These authors contributed equally to this work.
Correspondence should be addressed to Xiaowei Zhuang zhuang@chemistry.harvard.edu We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.
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