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Brief Communication

Nature Methods 3, 27–29 (1 January 2006) | doi:10.1038/nmeth843

In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes

Wigard P Kloosterman , Erno Wienholds , Ewart de Bruijn , Sakari Kauppinen & Ronald H A Plasterk

MicroRNAs (miRNAs) are 20–23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development.