Nature Methods 3, 27 - 29 (2006)
Published online: 20 December 2005; | doi:10.1038/nmeth843
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probesWigard P Kloosterman1, Erno Wienholds1, Ewart de Bruijn1, Sakari Kauppinen2
& Ronald H A Plasterk11
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands. 2
Wilhelm Johannsen Centre for Functional Genome Research, Institute of Medical Biochemistry and Genetics, University of Copenhagen, Blegdamsvej 3, DK-2200, Denmark.
Correspondence should be addressed to Ronald H A Plasterk plasterk@niob.knaw.nl MicroRNAs (miRNAs) are 20–23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
