Nature Methods 3, 23 - 25 (2006)
Published online: 20 December 2005; | doi:10.1038/nmeth816
Fluorescent sensors for rapid monitoring of intracellular cGMPViacheslav O Nikolaev1, Stepan Gambaryan2
& Martin J Lohse11
Institute of Pharmacology and Toxicology, Versbacher Str. 9, D-97078 Würzburg, Germany. 2
Institute of Clinical Biochemistry and Pathobiochemistry, Joseph Schneider Str. 2, D-97078 Würzburg, Germany.
Correspondence should be addressed to Martin J Lohse lohse@toxi.uni-wuerzburg.de Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and spatial resolution attained with the new sensors is superior to that of existing techniques, and permits direct recording and imaging of rapid cGMP-signaling events.
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