Nature Methods
2, 663 - 665 (2005)
Published online: 23 August 2005; | doi:10.1038/nmeth786
Gene expression profiling in single cells within tissuePaola Capodieci1, Michael Donovan1, Heidi Buchinsky1, Yusuf Jeffers1, Carlos Cordon-Cardo2, William Gerald2, Jon Edelson1, Shailesh M Shenoy3
& Robert H Singer31
Aureon Laboratories, 28 Wells Avenue, Yonkers, New York 10701, USA. 2
Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA. 3
Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
Correspondence should be addressed to Paola Capodieci paola.capodieci@aureon.com or Robert H Singer rhsinger@aecom.yu.edu We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
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