 | Figure 1
Nature Methods
2, 580 - 582 (2005)
doi:10.1038/nmeth0805-580
Insect transgenesis by site-specific recombinationErnst A Wimmer | | | | Figure 1. Position effect-caused variability of transgene expression can be overcome by site-specific recombination
(a) Randomly integrated transposon-mediated transgene insertions are subject to position effects. (b) Integration by site-specific recombination (indicated by the red cross) using one single target site is defied by efficient excision. Integration of the complete plasmid (transgene and backbone; thin line) is mediated by fC31 integrase, which uses the two different recombination sites attB and attP. (c) RMCE using heterospecific recombinase target sites integrates a transgene without the plasmid backbone at a chromosomal target site.
Katie Ris |
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