Nature Methods
2, 583 - 585 (2005)
Published online: 21 July 2005; | doi:10.1038/nmeth775
Site-specific transgenesis by Cre-mediated recombination in DrosophilaAdam Oberstein1, 2, 3, Adam Pare1, 2, Leah Kaplan1
& Stephen Small11
Department of Biology, New York University, 100 Washington Square East, New York, New York 10003, USA. 2
These authors contributed equally to this work. 3
Present address: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
Correspondence should be addressed to Stephen Small stephen.small@nyu.edu Transposons such as P elements are routinely used to stably transfer exogenous DNA (transgenes) into the Drosophila genome. Transgene insertion events, however, are essentially random and are subject to 'position effects' from nearby endogenous regulatory elements. Here we describe a microinjection-based system that uses Cre-mediated recombination to insert transgenes into precise genomic 'landing sites'. The system is simple and efficient, and will permit precise comparisons between multiple transgenic constructs.
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