Nature Methods
2, 587 - 589 (2005)
Published online: 21 July 2005; | doi:10.1038/nmeth774
Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptidesRobert J Beynon1, Mary K Doherty1, Julie M Pratt1
& Simon J Gaskell21
Department of Veterinary Preclinical Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZJ, UK. 2
Michael Barber Centre for Mass Spectrometry, School of Chemistry, University of Manchester, Manchester M13 9PL, UK.
Correspondence should be addressed to Robert J Beynon r.beynon@liv.ac.uk Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope−labeled analogs. The principal limitation to widespread implementation of this approach is the availability of standard signature peptides in accurately known amounts. We report the successful design and construction of an artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gallus gallus) skeletal muscle proteins and features for quantification and purification.
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