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Volume 2 Issue 5, May 2005

Image of DNA hybridized to a long oligonucleotide microarray. Photograph courtesy of Brenda Weis; artistic adaptation by Erin Boyle. Articles p337, 345 and 351

Editorial

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Research Highlights

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News & Views

  • Since the introduction of microarray technology into the biologist's arsenal, there have been concerns about the reproducibility of experimental results obtained using different microarray platforms. In this issue, three articles address this point, and show that with carefully designed and controlled experiments using standardized protocols and data analyses, reproducibility across platforms is much better than previously shown.

    • Gavin Sherlock
    News & Views
  • Mass spectrometry is reaching new heights monitoring changes in protein folding that occur as a result of a phage infection that commandeers the host's protein-folding machinery.

    • Carol V Robinson
    News & Views
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Perspective

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Article

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Protocol

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Technology Feature

  • The culture of animal cells is key to much of basic research today and an important starting point for therapeutic applications. But each cell type has its own quirks. Some cells are happy with most media and protocols, but others can become the bane of a scientist's existence with their seemingly inexplicable needs. Caitlin Smith reports.

    • Caitlin Smith
    Technology Feature
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Advertising Feature: Application Note

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Classic Protocol

  • Preparations of RNA containing an mRNA of interest are hybridized to a complementary single-stranded DNA probe. At the end of the reaction period, nuclease S1 is used to degrade unhybridized regions of the probe, and the surviving DNA-RNA hybrids are then separated by gel electrophoresis and visualized by either autoradiography or Southern hybridization. The method can be used to quantify RNAs, to map the positions of introns and to identify the locations of 5′ and 3′ ends of mRNAs on cloned DNA templates1,2,3.

    Classic Protocol
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Erratum

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Corrigendum

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