 | Figure 2
Nature Methods
2, 201 - 206 (2005)
Published online: 17 February 2005; | doi:10.1038/nmeth739
Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acidNobumasa Hino, Yuko Okazaki, Takatsugu Kobayashi, Akiko Hayashi, Kensaku Sakamoto
& Shigeyuki Yokoyama | | | | Figure 2. Analysis of the interactions between Grb2 and the EGF receptor by coimmunoprecipitation.
The wild-type Grb2 or Grb2(pBpa111) was expressed together with the EGF receptor in CHO cells, either treated with EGF or not treated. Ab, antibody. (a) A 20- g aliquot of the cell lysate was analyzed with the antibody against the EGF receptor. (b−d) An immunoprecipitate with the anti-Flag antibody from 1.0 mg of the cell lysate was probed with antibodies against the EGF receptor (b), phospho-Tyr1068 (c) and the Flag tag (d).
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