 | Figure 1
Nature Methods
2, 201 - 206 (2005)
Published online: 17 February 2005; | doi:10.1038/nmeth739
Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acidNobumasa Hino, Yuko Okazaki, Takatsugu Kobayashi, Akiko Hayashi, Kensaku Sakamoto
& Shigeyuki Yokoyama | | | | Figure 1. Site-specific incorporation of pBpa in the Grb2 SH2 domain.
(a) Chemical structure of a pBpa residue. The crystal structure (stereo view) of the complex between the Grb2 SH2 domain and a peptide inhibitor with the sequence Glu−phospho-Tyr−Ile−Asn−Gln (in green), which corresponds to residues 1067−1071 of the EGF receptor20. (b) pTyr, phosphotyrosine. Residue 111 is shown in magenta and residues 104, 119, 121 and 143 in cyan. (c) Illustration of a photo-cross-link between pBpa in position 111 of Grb2 and a bound protein. (d) The crystal structure (stereo view) of the complex between the Grb2 SH2 and the peptide inhibitor, in which residues 103−112 on the  D and D' strands20 are shown in orange.
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