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Nature Methods 2, 87 (1 February 2005) | doi:10.1038/nmeth0205-87

A better way to pick a plum

Michael Eisenstein

Working with naturally mutation-prone cell lines could spare researchers a lot of the bother involved in directed evolution studies; Roger Tsien and colleagues demonstrate how letting cells do all the grunt work of sequence modification allowed the group to develop mPlum, a robust and substantially red-shifted red fluorescent protein (RFP) variant.