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| A series of review articles and other content discussing the principles of fluorescence and different imaging modalities along with practical advice. This focus is available online through January 2006 thanks to our principal sponsor Carl Zeiss Microscopy and supporting sponsor Intelligent Imaging Solutions, Inc. |
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| Editorial | Top |
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Seeing is believing p889
doi:10.1038/nmeth1205-889
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(30K)
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| Research Highlights | Top |
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Making ES cells 'ethically sound' p891
Michael Eisenstein
doi:10.1038/nmeth1205-891
Abstract | Full text | PDF
(59K)
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MicroRNA: the perfect host pp892 - 893
Nicole Rusk
doi:10.1038/nmeth1205-892a
Abstract | Full text | PDF
(49K)
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Teaching cell biologists how to count pp892 - 893
Allison Doerr
doi:10.1038/nmeth1205-892b
Abstract | Full text | PDF
(49K)
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News in Brief p893
doi:10.1038/nmeth1205-893
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(30K)
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'Computational evolution' offers riboswitch solution p894
Michael Eisenstein
doi:10.1038/nmeth1205-894
Abstract | Full text | PDF
(28K)
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Decking out your DNA p896
Michael Eisenstein
doi:10.1038/nmeth1205-896a
Abstract | Full text | PDF
(30K)
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A biological tool with chemical applications p896
Allison Doerr
doi:10.1038/nmeth1205-896b
Abstract | Full text | PDF
(30K)
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| News and Views | Top |
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Frog transgenesis made simple pp897 - 898
Stuart J Smith
& Timothy J Mohun
doi:10.1038/nmeth1205-897
Abstract | Full text | PDF
(70K)
See also: Article by Allen & Weeks |
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FlAsHlights on bacterial virulence proteins pp898 - 899
Luís Jaime Mota
& David W Holden
doi:10.1038/nmeth1205-898
Abstract | Full text | PDF
(463K)
See also: Article by Enninga et al. |
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| Foreword | Top |
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Focus on Fluorescence Imaging
Focus on fluorescence imaging p901
Daniel Evanko
doi:10.1038/nmeth1205-901
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(129K)
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| Commentary | Top |
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Focus on Fluorescence Imaging
Fluorescence microscopy today pp902 - 904
Rafael Yuste
doi:10.1038/nmeth1205-902
Abstract | Full text | PDF
(52K)
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| Perspective | Top |
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Focus on Fluorescence Imaging
A guide to choosing fluorescent proteins pp905 - 909
Nathan C Shaner, Paul A Steinbach
& Roger Y Tsien
Published online: 18 November 2005 | doi:10.1038/nmeth819
Abstract | Full text | PDF
(70K)
| Supplementary Information |
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Focus on Fluorescence Imaging
Fluorescence microscopy pp910 - 919
Jeff W Lichtman
& José-Angel Conchello
Published online: 18 November 2005 | doi:10.1038/nmeth817
Abstract | Full text | PDF
(499K)
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Focus on Fluorescence Imaging
Optical sectioning microscopy pp920 - 931
José-Angel Conchello
& Jeff W Lichtman
Published online: 18 November 2005 | doi:10.1038/nmeth815
Abstract | Full text | PDF
(589K)
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Focus on Fluorescence Imaging
Deep tissue two-photon microscopy pp932 - 940
Fritjof Helmchen
& Winfried Denk
Published online: 18 November 2005 | doi:10.1038/nmeth818
Abstract | Full text | PDF
(574K)
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Focus on Fluorescence Imaging
Fiber-optic fluorescence imaging pp941 - 950
Benjamin A Flusberg, Eric D Cocker, Wibool Piyawattanametha, Juergen C Jung, Eunice L M Cheung
& Mark J Schnitzer
Published online: 18 November 2005 | doi:10.1038/nmeth820
Abstract | Full text | PDF
(586K)
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| Brief Communications | Top |
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Mutation discovery in bacterial genomes: metronidazole resistance in Helicobacter pylori pp951 - 953
Thomas J Albert, Daiva Dailidiene, Giedrius Dailide, Jason E Norton, Awdhesh Kalia, Todd A Richmond, Michael Molla, Jaz Singh, Roland D Green
& Douglas E Berg
Published online: 18 November 2005 | doi:10.1038/nmeth805
Abstract | Full text | PDF
(245K)
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Positional proteomics: selective recovery and analysis of N-terminal proteolytic peptides pp955 - 957
Lucy McDonald, Duncan H L Robertson, Jane L Hurst
& Robert J Beynon
Published online: 18 November 2005 | doi:10.1038/nmeth811
Abstract | Full text | PDF
(452K)
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| Articles | Top |
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Secretion of type III effectors into host cells in real time pp959 - 965
Jost Enninga, Joëlle Mounier, Philippe Sansonetti
& Guy Tran Van Nhieu
Published online: 18 November 2005 | doi:10.1038/nmeth804
Abstract | Full text | PDF
(402K)
| Supplementary Information
See also: News and Views by Mota & Holden |
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High-throughput screening of effective siRNAs from RNAi libraries delivered via bacterial invasion pp967 - 973
Hui-Fen Zhao, Denis L'Abbé, Normand Jolicoeur, Meiqun Wu, Zhen Li, Zhenbao Yu
& Shi-Hsiang Shen
Published online: 18 November 2005 | doi:10.1038/nmeth812
Abstract | Full text | PDF
(965K)
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Transgenic Xenopus laevis embryos can be generated using  C31 integrase pp975 - 979
Bryan G Allen
& Daniel L Weeks
Published online: 18 November 2005 | doi:10.1038/nmeth814
Abstract | Full text | PDF
(262K)
See also: News and Views by Smith & Mohun |
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| Protocol | Top |
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Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells pp981 - 987
Patricia Wadsworth, Nasser M Rusan, U Serdar Tulu
& Carey Fagerstrom
doi:10.1038/nmeth1205-981
Full text | PDF
(327K)
| Supplementary Information |
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| Technology Feature | Top |
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The SNPs are down: genotyping for the rest of us pp989 - 994
Alan Dove
doi:10.1038/nmeth1205-989
Abstract | Full text | PDF
(405K)
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| Classic Protocol | Top |
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A look back: getting the message p996
Michael Eisenstein
doi:10.1038/nmeth1205-996
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(27K)
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Northern blotting: transfer of denatured RNA to membranes pp997 - 998
doi:10.1038/nmeth1205-997
Abstract | Full text | PDF
(31K)
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| Application Note | Top |
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High-content screening in oncology using fluorescence microplate cytometry
Wayne P Bowen
& Sarah L Payne
doi:10.1038/nmeth824
Abstract | Full text | PDF
(326K)
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