Nature Methods 2, 955 - 957 (2005)
Published online: 18 November 2005; | doi:10.1038/nmeth811
Positional proteomics: selective recovery and analysis of N-terminal proteolytic peptidesLucy McDonald1, Duncan H L Robertson1, Jane L Hurst2
& Robert J Beynon11
Protein Function Group, Faculty of Veterinary Science, University of Liverpool, Crown Street, Liverpool L69 7ZJ, UK. 2
Mammalian Behavior and Evolution Group, Faculty of Veterinary Science, University of Liverpool, Crown Street, Liverpool L69 7ZJ, UK.
Correspondence should be addressed to Robert J Beynon r.beynon@liv.ac.uk Bottom-up proteomics is the analysis of peptides derived from single proteins or protein mixtures, and because each protein generates tens of peptides, there is scope for controlled reduction in complexity. We report here a new strategy for selective isolation of the N-terminal peptides of a protein mixture, yielding positionally defined peptides. The method is tolerant of several fragmentation methods, and the databases that must be searched are substantially less complex.
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