Nature Methods
2, 763 - 769 (2005)
Published online: 22 September 2005; | doi:10.1038/nmeth795
Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon systemVincent W Keng, Kojiro Yae, Tomoko Hayakawa, Sumi Mizuno, Yoshihiro Uno, Kosuke Yusa, Chikara Kokubu, Taroh Kinoshita, Keiko Akagi, Nancy A Jenkins, Neal G Copeland, Kyoji Horie
& Junji Takeda Supplementary Fig. 1 (pdf 76K)
Examples of two phenotypic screens generated by SB transposon system. Supplementary Fig. 2 (pdf 88K)
Determining the exact location of IR3 donor site on chromosome 12. Supplementary Fig. 3 (pdf 216K)
Gene trap and region-specific saturation mutagenesis using the SB transposon system for IF2 donor mouse. Supplementary Fig. 4 (pdf 92K)
Detection of new transcripts using the SB transposon system. Supplementary Fig. 5 (pdf 140K)
Screen shot of the UCSC Mouse Genome Browser Version mm4 with our SB database overview of transposon insertion sites for IR3 and IF2 donor mice. Supplementary Table 1 (pdf 56K)
List of primers used in the nested-PCR. Supplementary Methods (pdf 60K)
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