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Volume 14 Issue 3, March 2017

CROP-seq genetic screening: CRISPR-edited single cells are encapsulated in bead-containing emulsion droplets for single-cell RNA-seq. Cover design by P. Datlinger and N. Winhofer. Article, p297

Editorial

  • Careful construction of reference lists is the responsibility of every scientist.

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  • An engineered approach to study gene regulation, and why home is where the heart is.

    • Vivien Marx
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  • A P value measures a sample's compatibility with a hypothesis, not the truth of the hypothesis.

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    • Martin Krzywinski
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Methods in Brief

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Research Highlights

  • Low-energy holography enables imaging single proteins and protein complexes.

    • Zachary J Lapin
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Commentary

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News & Views

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Review Article

  • This Review discusses key aspects of experimental design to evaluate intergenerational and transgenerational epigenetic inheritance. It describes advantages of patrilineal and matrilineal design, and it offers advice for selecting animals for breeding and for determining the weaning scheme and group size.

    • Johannes Bohacek
    • Isabelle M Mansuy
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