Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Removing roadblocks to deep sequencing of modified RNAs

By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Removing methylated nucleotides to sequence tRNAs.

References

  1. Zheng, G. et al. Nat. Methods 12, 835–837 (2015).

    Article  CAS  Google Scholar 

  2. Cozen, A.E. et al. Nat. Methods 12, 879–884 (2015).

    Article  CAS  Google Scholar 

  3. Wilusz, J.E. Wiley Interdiscip. Rev. RNA 6, 453–470 (2015).

    Article  CAS  Google Scholar 

  4. Gingold, H. et al. Cell 158, 1281–1292 (2014).

    Article  CAS  Google Scholar 

  5. Pavon-Eternod, M., Gomes, S., Rosner, M.R. & Pan, T. RNA 19, 461–466 (2013).

    Article  CAS  Google Scholar 

  6. Anderson, P. & Ivanov, P. FEBS Lett. 588, 4297–4304 (2014).

    Article  CAS  Google Scholar 

  7. Wang, Z., Gerstein, M. & Snyder, M. Nat. Rev. Genet. 10, 57–63 (2009).

    Article  CAS  Google Scholar 

  8. Machnicka, M.A. et al. Nucleic Acids Res. 41, D262–D267 (2013).

    Article  CAS  Google Scholar 

  9. Trewick, S.C., Henshaw, T.F., Hausinger, R.P., Lindahl, T. & Sedgwick, B. Nature 419, 174–178 (2002).

    Article  CAS  Google Scholar 

  10. Mohr, S. et al. RNA 19, 958–970 (2013).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

J.E.W. is supported by US National Institutes of Health grant R00-GM104166 and the Rita Allen Foundation.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jeremy E Wilusz.

Ethics declarations

Competing interests

The author declares no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wilusz, J. Removing roadblocks to deep sequencing of modified RNAs. Nat Methods 12, 821–822 (2015). https://doi.org/10.1038/nmeth.3516

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.3516

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing