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InnoStamp 40 and InnoScan 1100AL: a complete automated platform for microstructured cell arrays

Journal name:
Nature Methods
Volume:
12
Year published:
Published online

Abstract

Microstructured cell arrays are obtained by patterning of extracellular matrix (ECM) components on a substrate to control cell spread. Because such arrays are of interest to different fields related to cell biology, automatic production and parallelized assays are needed. With complete automation of ECM 2D patterning and whole-slide image acquisition, the InnoStamp 40 and the InnoScan 1100AL are ideal tools for high-throughput assays for the study of cell fate decisions in the presence of external stimuli such as drugs, toxins or mechanical stress.

At a glance

Figures

  1. Automatic microstructured cell array process used in InnoStamp 40[trade].
    Figure 1: Automatic microstructured cell array process used in InnoStamp 40.

    (a) A magnetic PDMS stamp containing the features is used as a transfer stamp. (b) The magnetic PDMS stamp is inked with ECM components. (c) The stamp is air dried. (d) The magnetic stamp is put in contact with the substrate. (e) ECM components are transferred to the substrate according to the stamp pattern. (f) The slide is treated with a non-adhesive molecule such as PLL-g-PEG. (g) The antifouling molecule covers the regions around the ECM pattern. (h) The InnoStamp 40 automated microcontact printer together with some examples of ECM patterned slides. The spot diameter and line width range from 50 to 150 μm. (i) Cells cultured in micropatterned slides; after 24 h cells selectively attach to sites containing ECM molecules. (j) After fluorescence staining, cells are detected with the InnoScan 1100AL scanner. A whole-slide image at a resolution of 0.5 μm per pixel is shown.

  2. ECM cell-spread patterning.
    Figure 2: ECM cell-spread patterning.

    PC3-GFP cells were cultured on an array of printed lines of collagen surrounded by antifouling PLL-g-PEG zones. Nuclei were labeled with DRAQ5 dye detected in the 635-nm channel (magenta). GFP (excitation/emission wavelength: 488/509 nm) was detected in the 488-nm channel (blue). (a) A whole-slide image scanned at a resolution of 0.5 μm per pixel. (b) Zoomed view of the image in a.

  3. The Innopsys InnoStamp 40[trade] uses permanent magnets to automate the full microcontact printing process.
    Figure 3: The Innopsys InnoStamp 40 uses permanent magnets to automate the full microcontact printing process.

References

  1. LeDuc, P., Ostuni, E., Whitesides, G. & Ingber, D. Use of micropatterned adhesive surface for control of cell behavior. Methods Cell Biol. 69, 385401 (2002).
  2. Soen, Y., Mori, A., Palmer, T.D. & Brown, P.O. Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments. Mol. Syst. Biol. 2, 37 (2006).
  3. Cau, J.C., Lafforgue, L., Nogues, M., Lagraulet, A. & Paveau, V. Magnetic field assisted microcontact printing: a new concept of fully automated and calibrated process. Microelec. Eng. 110, 207214 (2013).

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Author information

Affiliations

  1. INNOPSYS, Carbonne, France.

    • Adriana Lagraulet &
    • Benjamin Berteloite
  2. Biosoft-CNRS, LAAS, Toulouse, France.

    • Julie Foncy,
    • Aurore Esteve,
    • Laurent Malaquin &
    • Christophe Vieu
  3. CNRS, LAAS, Toulouse, France.

    • Marie-Charline Blatche
  4. Université de Toulouse, INSA, LAAS, Toulouse, France.

    • Christophe Vieu

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Disclaimer

This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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