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Volume 12 Issue 3, March 2015

Chemical structures of bright organic fluorophores containing a novel azetidine group. The colors of the lightbulb outlines match the emission maxima of the fluorophores.Original artwork by Luke Lavis, Howard Hughes Medical Institute/Janelia Farm Research Campus. Cover by Erin Dewalt. Article p244.

Editorial

  • The Roadmap Epigenomics Project generates valuable resources and tools and also highlights questions that still need addressing.

    Editorial

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This Month

  • Developing biosensors takes patience and running through the snow.

    • Vivien Marx
    This Month
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Correspondence

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Research Highlights

  • Expansion microscopy uses enlarged samples for high-resolution imaging with conventional microscopes.

    • Rita Strack
    Research Highlights
  • Swept, confocally aligned planar excitation imaging is a fast light-sheet microscopy technique that can be applied to live samples such as behaving animals.

    • Nina Vogt
    Research Highlights
  • Modeling cell-cycle state from the transcriptional profiles of single cells can improve the ability to group cells by function.

    • Tal Nawy
    Research Highlights
  • Guide RNAs can serve as scaffolds to flexibly recruit different effector modules to specific genomic loci.

    • Natalie de Souza
    Research Highlights
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Methods in Brief

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Tools in Brief

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Research Highlights

  • Three groups report key steps toward nanometer-scale magnetic resonance imaging using nitrogen-vacancy centers in diamond.

    • Allison Doerr
    Research Highlights
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Commentary

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Technology Feature

  • Blinking and photobleaching of fluorophores cause challenges in a whole range of imaging experiments. Here are some ways researchers are approaching fluorophore photostability.

    • Vivien Marx

    Collection:

    Technology Feature
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