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Extracellular matrix patterned in a mesh consisting of 20-μm-wide stripes of laminin (green) and fibronectin (purple) spaced 20 μm apart and overlaid conformally on highaspect ratio microridges. Image by Q. Jallerat and J.M. Szymanski, Carnegie Mellon University. Article p134.
An engineered dCas9–guide RNA complex containing three different transcriptional activation domains achieves consistently high expression of endogenous genes in a genome-wide screen.
A Perspective on the open-source and open-development software project Bioconductor provides an overview for prospective users and developers dealing with high-throughput data in genomics and molecular biology.
A DNA nanoswitch platform enables inexpensive kinetic and thermodynamic analysis of a range of biomolecular interactions using a simple gel electrophoresis readout.
An improved analysis approach for single-wavelength anomalous diffraction (SAD)-based macromolecular X-ray structure determination expands the applicability of this phasing method for macromolecules with weak anomalous signals.
A native-SAD (single-wavelength anomalous diffraction) data collection strategy enables phasing using anomalous signal from a single native crystal, facilitating straightforward macromolecular X-ray structure determination.
Snap-tag reporter mice allow flexible yet efficient targeting of chemical indicators to genetically labeled cells in vivo. With this strategy, cells can either be labeled fluorescently or ablated using the same reporter.
Here, the authors present an approach for the simultaneous optogenetic manipulation and recording of neural activity at cellular resolution using two-photon microscopy and apply their strategy in the mouse barrel cortex.
QTI-seq (quantitative translation initiation sequencing) maps the position of the start codon and thus allows the determination of initiation efficiency in response to various stimuli, such as starvation, in cell culture and in vivo.
The prix fixe strategy and software uses cofunction networks to identify causal genes among candidate genes in disease-associated loci from genome-wide association studies.