Video 1: Adaptive optics (AO) over a large volume in the living zebrafish brain

FromRapid adaptive optical recovery of optimal resolution over large volumes

Journal name:
Nature Methods
Volume:
11,
Pages:
625–628
Year published:
DOI:
doi:10.1038/nmeth.2925
Received
Accepted
Published online
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Two photon 3D image of a membrane-labeled subset of neurons in the brain of a living zebrafish embryo, 72 hours post fertilization. This 240 x 240 x 270 μm3 imaging volume consists of 19,584 corrective subvolumes, each 30 x 30 x 1.05 μm3 in extent. Zooming deep in the mid-brain, the individual neuronal processes, unresolved without AO, become distinct after correction.

Additional data