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Art and artifacts in single-molecule localization microscopy: beyond attractive images

Nature Methods volume 11, pages 235–238 (2014)Cite this article

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Single-molecule super-resolution techniques emerged only several years ago but have revolutionized fluorescence microscopy of cellular structures. We discuss some key principles of these techniques, point out pitfalls, highlight recent developments and identify opportunities for the future.

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Figure 1: Cellular structures can serve as a benchmark for calculating the spatial resolution.

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Acknowledgements

This work was supported by the Systems Biology Initiative (FORSYS) of the Bundesministerium für Bildung und Forschung and by the German Science Foundation (EXC 115).

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Authors and Affiliations

  1. Ulrike Endesfelder and Mike Heilemann are in the Single Molecule Biophysics Group, Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Frankfurt, Germany.,

    Ulrike Endesfelder & Mike Heilemann

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  1. Ulrike Endesfelder
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  2. Mike Heilemann
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Correspondence to Ulrike Endesfelder or Mike Heilemann.

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Endesfelder, U., Heilemann, M. Art and artifacts in single-molecule localization microscopy: beyond attractive images. Nat Methods 11, 235–238 (2014). https://doi.org/10.1038/nmeth.2852

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