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Genome editing 101: let's go digital

Nature Methods volume 11, pages 248–249 (2014)Cite this article

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An approach is described that simplifies the isolation of rare human pluripotent stem cells engineered to carry precise disease-relevant mutations.

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Figure 1: Isolation of rare engineered mutant human pluripotent stem cell clones by iterative subcloning and ddPCR screening.

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Authors and Affiliations

  1. Ryan Forster and Dirk Hockemeyer are in the Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.,

    Ryan Forster & Dirk Hockemeyer

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  1. Ryan Forster
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  2. Dirk Hockemeyer
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Correspondence to Dirk Hockemeyer.

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The authors declare no competing financial interests.

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Forster, R., Hockemeyer, D. Genome editing 101: let's go digital. Nat Methods 11, 248–249 (2014). https://doi.org/10.1038/nmeth.2859

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