Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
A llama. Llamas produce single chain antibodies that are used to generate small antigen-binding proteins known as nanobodies. Original image by Capralogics, Inc. Article p1253
The 2014 Nobel Prize in Chemistry has been awarded in recognition of super-resolution fluorescence microscopy. To fully realize the tremendous promise of these methods for biological discovery, there are still challenges to be overcome.
The idea of using hybrid proteins containing transcription factor domains to analyze protein-protein interactions was described in 1989. Over the past 25 years, this method has begun to reveal the complex protein networks that underlie cellular behavior.
Tissue samples scatter light and shut out a deeper gaze. Tissue-clearing agents have changed that, but scientists must consider optimizing the optics when using these methods.
Avoiding nonspecific binding is essential for performing fluorescence microscopy–based analyses of single molecules tethered to surfaces. A dichlorodimethylsilane–Tween-20–passivated surface provides a useful alternative to the standard poly(ethylene glycol) surface for single-molecule studies.
Quantum dot–coated pipettes facilitate targeted electrophysiological recordings in deep brain tissue because they are readily visible owing to the strong two-photon fluorescence of the quantum dots.
This paper investigates the stress levels and behavior of wild animals when approached by remote-operated vehicles versus humans for data collection in field research
Cell-based reporters for dopamine and norepinephrine allow real-time measurements of these neurotransmitters in vivo with high specificity. They can address the temporal and spatial dynamics of volume neurotransmission in behaving animals.
This paper presents an efficient method for generating nanobodies with high affinity and high specificity. In addition, a collection of nanobodies specific for GFP or mCherry that resulted from this work is described.