Entering the era of single-cell transcriptomics in biology and medicine

Journal name:
Nature Methods
Volume:
11,
Pages:
22–24
Year published:
DOI:
doi:10.1038/nmeth.2764
Published online

Recent technical advances have enabled RNA sequencing (RNA-seq) in single cells. Exploratory studies have already led to insights into the dynamics of differentiation, cellular responses to stimulation and the stochastic nature of transcription. We are entering an era of single-cell transcriptomics that holds promise to substantially impact biology and medicine.

References

  1. Novick, A. & Weiner, M. Proc. Natl. Acad. Sci. USA 43, 553566 (1957).
  2. Brady, G., Barbara, M. & Iscove, N.N. Methods Mol. Biol. 2, 1725 (1990).
  3. Eberwine, J. et al. Proc. Natl. Acad. Sci. USA 89, 30103014 (1992).
  4. Islam, S. et al. Nat. Protoc. 7, 813828 (2012).
  5. Tang, F. et al. Nat. Protoc. 5, 516535 (2010).
  6. Picelli, S. et al. Nat. Protocols, doi:10.1038/nprot.2014.006 (2 January 2014).
  7. Yan, L. et al. Nat. Struct. Mol. Biol. 20, 11311139 (2013).
  8. Tang, F. et al. Cell Stem Cell 6, 468478 (2010).
  9. Ramsköld, D. et al. Nat. Biotechnol. 30, 777782 (2012).
  10. Islam, S. et al. Genome Res. 21, 11601167 (2011).
  11. Picelli, S. et al. Nat. Methods 10, 10961098 (2013).
  12. Kivioja, T. et al. Nat. Methods 9, 7274 (2012).
  13. Hashimshony, T., Wagner, F., Sher, N. & Yanai, I. Cell Rep 2, 666673 (2012).
  14. Shapiro, E., Biezuner, T. & Linnarsson, S. Nat. Rev. Genet. 14, 618630 (2013).
  15. Dominissini, D. et al. Nature 485, 201206 (2012).
  16. Raj, A. & van Oudenaarden, A. Cell 135, 216226 (2008).
  17. Chubb, J.R., Trcek, T., Shenoy, S.M. & Singer, R.H. Curr. Biol. 16, 10181025 (2006).
  18. Wills, Q.F. et al. Nat. Biotechnol. 31, 748752 (2013).
  19. McDavid, A. et al. Bioinformatics 29, 461467 (2013).
  20. Mercer, T.R. et al. Nat. Biotechnol. 30, 99104 (2012).
  21. Wang, E.T. et al. Nature 456, 470476 (2008).
  22. Shalek, A.K. et al. Nature 498, 236240 (2013).
  23. Sandberg, R., Neilson, J.R., Sarma, A., Sharp, P.A. & Burge, C.B. Science 320, 16431647 (2008).
  24. Mayr, C. & Bartel, D.P. Cell 138, 673684 (2009).
  25. Rhodes, D.R. et al. Proc. Natl. Acad. Sci. USA 101, 93099314 (2004).
  26. Golub, T.R. et al. Science 286, 531537 (1999).
  27. Dalerba, P. et al. Nat. Biotechnol. 29, 11201127 (2011).
  28. Gerlinger, M. et al. N. Engl. J. Med. 366, 883892 (2012).
  29. Yu, M. et al. Nature 487, 510513 (2012).
  30. Vogelstein, B. et al. Science 339, 15461558 (2013).
  31. Kim, H.D., Shay, T., O'Shea, E.K. & Regev, A. Science 325, 429432 (2009).

Download references

Author information

Affiliations

  1. Rickard Sandberg is at the Ludwig Institute for Cancer Research, Stockholm, Sweden, and Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

Competing financial interests

The author declares no competing financial interests.

Corresponding author

Correspondence to:

Author details

Additional data