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OpenSpinMicroscopy: an open-source integrated microscopy platform

Nature Methods volume 10, pages 599–600 (2013)Cite this article

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To the Editor:

Light-sheet microscopy has recently emerged as the technique of choice for fast, high-resolution three-dimensional (3D) imaging of whole organisms, one that also features low photodamage1,2. Unlike confocal or multiphoton systems, light sheet–based microscopes can easily provide a combination of multiple views by rotating the sample. Multiview imaging solves the problems caused by light scattering, shadowing and anisotropy that are inherent to uniaxial optics. Sample rotation is also a fundamental aspect of optical tomography techniques3. However, image-acquisition and microscope-control software is not, by default, prepared to handle stage rotation.

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Figure 1: Multimodality imaging with OpenSpinMicroscopy.

References

  1. Pastrana, E. Nat. Methods 9, 37 (2011).

    Article  Google Scholar 

  2. Huisken, J. & Stainier, D.Y.R. Development 136, 1963–1975 (2009).

    Article  CAS  Google Scholar 

  3. Sharpe, J. et al. Science 296, 541–545 (2002).

    Article  CAS  Google Scholar 

  4. Edelstein, A., Amodaj, N., Hoover, K., Vale, R. & Stuurman, N. Curr. Protoc. Mol. Biol. 92, 14.20 (2010).

    Google Scholar 

  5. Keller, P.J. et al. Nat. Methods 7, 637–642 (2010).

    Article  CAS  Google Scholar 

  6. Preibisch, S., Saalfeld, S., Schindelin, J. & Tomancak, P. Nat. Methods 7, 418–419 (2010).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank C. Gonzalez (Institute for Research in Biomedicine Barcelona) for the use of his line of flies; M. Bettencourt-Dias and A. Jurberg (Instituto Gulbenkian de Ciência) for providing flies and mutant mouse embryos; and A.C. Borges (Centro de Estudos de Doenças Crónicas) for providing zebrafish embryos. E.J.G. acknowledges support from the Fundação para a Ciência e a Tecnologia grant SFRH/BPD/80717/2011. G.G.M. acknowledges the support of the Microscopy Unit of Faculdade de Ciências da Universidade de Lisboa.

Author information

Authors and Affiliations

  1. Instituto Gulbenkian de Ciência, Oeiras, Portugal

    Emilio J Gualda, Tiago Vale, Pedro Almada, José A Feijó & Nuno Moreno

  2. Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, Portugal

    José A Feijó

  3. Centro de Biologia Ambiental, Faculdade de Ciências da Universidade de Lisboa, Lisbon, Portugal

    Gabriel G Martins

Authors
  1. Emilio J Gualda
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  2. Tiago Vale
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  3. Pedro Almada
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  4. José A Feijó
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  5. Gabriel G Martins
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  6. Nuno Moreno
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Corresponding author

Correspondence to Emilio J Gualda.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–6, Supplementary Table 1 and Supplementary Notes 1–3 (PDF 862 kb)

DSLM time-lapse recording

Maximum-intensity projection of the development over 8 h of a zebrafish embryo (Tg(β-actin:HRAS-EGFP)) expressing GFP acquired with the OpenSpinMicroscopy plug-in. (MOV 4808 kb)

Multiple view fusion

Volume reconstruction obtained after the fusion of eight different views of a transgenic mouse embryo (FVB/N.Tie II-GFP) expressing GFP. (MOV 1498 kb)

OPT reconstruction of E14.5 mouse embryo

Series of sagittal sections of an E14.5 mouse embryo obtained after back-projection reconstruction of an OPT dataset into axial sections. The OPT dataset represents green autofluorescence and was acquired using the dedicated OPT setup with a 1× magnification lens. (MOV 1264 kb)

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Gualda, E., Vale, T., Almada, P. et al. OpenSpinMicroscopy: an open-source integrated microscopy platform. Nat Methods 10, 599–600 (2013). https://doi.org/10.1038/nmeth.2508

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