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Method of the Year 2016

Epitranscriptome analysis is our choice for Method of the Year 2016. A News Feature looks at the history of the field, from the first discoveries of RNA modifications in the 1960s to recent transcriptome-wide methods. A Review describes the strengths and weaknesses of these methods, and a Commentary discusses the functional importance of a particular modification in stem cells. Our choice of eight methods to watch highlights areas we think will be influential in 2017 and beyond.

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News Feature

Focus on Method of the Year 2016

Epitranscriptomics: mixed messages pp15 - 17

Michael Eisenstein

doi:10.1038/nmeth.4125


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Commentary

Focus on Method of the Year 2016

Reversible RNA modifications in meiosis and pluripotency pp18 - 22

Arne Klungland, John Arne Dahl, Gareth Greggains, Peter Fedorcsak & Adam Filipczyk

doi:10.1038/nmeth.4111


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Review

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Epitranscriptome sequencing technologies: decoding RNA modifications pp23 - 31

Xiaoyu Li, Xushen Xiong & Chengqi Yi

doi:10.1038/nmeth.4110


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Methods to Watch

Focus on Method of the Year 2016

Global metabolomics p32

Allison Doerr

doi:10.1038/nmeth.4112

The ability to measure the metabolome on a global scale lags behind other omics techniques.


Focus on Method of the Year 2016

Expansion microscopy p32

Rita Strack

doi:10.1038/nmeth.4113

The changing face of super-resolution imaging


Focus on Method of the Year 2016

CRISPR targets RNA p33

Nicole Rusk

doi:10.1038/nmeth.4114

Having revolutionized DNA editing, CRISPR turns to RNA.


Focus on Method of the Year 2016

Cryo-electron tomography p34

Allison Doerr

doi:10.1038/nmeth.4115

Cryo-electron tomography may facilitate in situ structural biology on a proteomic scale.


Focus on Method of the Year 2016

Capturing microbial interactions p35

Tal Nawy

doi:10.1038/nmeth.4117

New approaches will expose microbial dependencies and environmental interactions.


Focus on Method of the Year 2016

Faster brain imaging p34

Nina Vogt

doi:10.1038/nmeth.4118

Higher volumetric imaging rates shed light on the dynamics in neuronal networks.


Focus on Method of the Year 2016

How single cells do it p33

Tal Nawy

doi:10.1038/nmeth.4119

Single-cell sequencing is poised to elucidate how cells contribute to tissue function.


Focus on Method of the Year 2016

Organoid culture p35

Natalie de Souza

doi:10.1038/nmeth.4122

Ex vivo organoid culture could revolutionize biology, but variability must be understood.


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