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<i>Nature Methods</i> focus on mass spectrometry in proteomics—a user's guide
Contents Foreword Commentaries Reviews
Perspectives Glossary NPG Library Feedback
 NPG Library
 Protocols
Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site-specific stable isotope tagging
Hiroyuki Kaji, Yoshio Yamauchi, Nobuhiro Takahashi & Toshiaki Isobe
 
Abstract |  Full text |  PDF
In-gel digestion for mass spectrometric characterization of proteins and proteomes
Andrej Shevchenko, Henrik Tomas, Jan Havlis, Jesper V Olsen & Matthias Mann
 
Abstract |  Full text |  PDF
A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)
Shao-En Ong & Matthias Mann
 
Abstract |  Full text |  PDF
Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)—a general method for mapping sites of probe modification in proteomes
Eranthie Weerapana, Anna E Speers & Benjamin F Cravatt
 
Abstract |  Full text |  PDF
Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging
Daniela C Dieterich, Jennifer J Lee, A James Link, Johannes Graumann, David A Tirrell & Erin M Schuman
 
Abstract |  Full text |  PDF
Solid-phase extraction of N-linked glycopeptides
Yuan Tian, Yong Zhou, Sarah Elliott, Ruedi Aebersold & Hui Zhang
 
Abstract |  Full text |  PDF
Verification of automated peptide identifications from proteomic tandem mass spectra
David L Tabb, David B Friedman & Amy-Joan L Ham
 
Abstract |  Full text |  PDF
Highly selective enrichment of phosphorylated peptides using titanium dioxide
Tine E Thingholm, Thomas J D J�rgensen, Ole N Jensen & Martin R Larsen
 
Abstract |  Full text |  PDF
Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics
Brian Cox & Andrew Emili
 
Abstract |  Full text |  PDF
Protein profiling by the combination of two independent mass spectrometry techniques
Wei-Qiang Chen, Sung-Ung Kang & Gert Lubec
 
Abstract |  Full text |  PDF
Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry
Yuzuru Shiio & Ruedi Aebersold
 
Abstract |  Full text |  PDF
Unrestrictive identification of post-translational modifications through peptide mass spectrometry
Stephen Tanner, Pavel A Pevzner & Vineet Bafna
 
Abstract |  Full text |  PDF
In-gel stable isotope labeling for relative quantification using mass spectrometry
John M Asara, Xiang Zhang, Bin Zheng, Lisa A Maroney, Heather R Christofk, Ning Wu & Lewis C Cantley
 
Abstract |  Full text |  PDF
Liquid-based free-flow electrophoresis-reversed-phase HPLC: a proteomic tool
Robert L Moritz & Richard J Simpson
 
Full text |  PDF
 
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Nature Methods
ISSN: 1548-7091
EISSN: 1548-7105
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