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Light-sheet Microscopy

The use of a planar sheet of light for illumination in fluorescence microscopy allows researchers to image sample volumes faster than possible with other current methods, while limiting light dosage. This collection of articles provides a brief overview of this exciting methodology and the biological research applications that it makes possible.


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Editorial

Editorial

Sheet of light

Daniel Evanko


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Light-sheet Microscopy collection

Perspective

The third dimension bridges the gap between cell culture and live tissue

Francesco Pampaloni, Emmanuel G. Reynaud & Ernst H. K. Stelzer

Nature Reviews Molecular Cell Biology 8, 839—845 (2007)

doi:10.1038/nrm2236


Review

Optical sectioning microscopy with planar or structured illumination

Jerome Mertz

Nature Methods 8, 811—819 (2011)

doi:10.1038/nmeth.1709


Correspondence

Software for bead-based registration of selective plane illumination microscopy data

Stephan Preibisch, Stephan Saalfeld, Johannes Schindelin & Pavel Tomancak

Nature Methods 7, 418—419 (2010)

doi:10.1038/nmeth0610-418


News and Views

Omnidirectional microscopy

Michael Weber & Jan Huisken

Nature Methods 9, 656—657 (2012)

doi:10.1038/nmeth.2022


Research Articles

Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain

Hans-Ulrich Dodt, Ulrich Leischner, Anja Schierloh, Nina Jährling, Christoph Peter Mauch, Katrin Deininger, Jan Michael Deussing, Matthias Eder, Walter Zieglgänsberger & Klaus Becker

Nature Methods 4, 331—336 (2007)

doi:10.1038/nmeth1036


Deep and fast live imaging with two-photon scanned light-sheet microscopy

Thai V Truong, Willy Supatto, David S Koos, John M Choi & Scott E Fraser

Nature Methods 8, 757—762 (2011)

doi:10.1038/nmeth.1652


Whole-brain functional imaging at cellular resolution using light-sheet microscopy

Misha B Ahrens, Michael B Orger, Drew N Robson, Jennifer M Li & Philipp J Keller

Nature Methods 10, 413—420 (2013)

doi:10.1038/nmeth.2434


High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics

Benjamin Schmid, Gopi Shah, Nico Scherf, Michael Weber, Konstantin Thierbach, Citlali Pérez Campos, Ingo Roeder, Pia Aanstad & Jan Huisken

Nature Communications 4, Article number: 2207 (2013)

doi:10.1038/ncomms3207


Live-cell 3D super-resolution imaging in thick biological samples

Francesca Cella Zanacchi, Zeno Lavagnino, Michela Perrone Donnorso, Alessio Del Bue, Laura Furia, Mario Faretta & Alberto Diaspro

Nature Methods 9, 1047—1049 (2011)

doi:10.1038/nmeth.1744


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