RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations

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    Light-dependent variants of Cre, Dre and Flp enable targeted sparse or single-cell labeling in mouse and zebrafish.

    • Shenqin Yao
    • , Peng Yuan
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    • , Soumya Chatterjee
    • , Yun Wang
    • , Tanya L. Daigle
    • , Bosiljka Tasic
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    • , Qingming Luo
    • , Shaoqun Zeng
    • , Andrew Curtright
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    • , Anat Kahan
    • , Viviana Gradinaru
    • , Radosław Chrapkiewicz
    • , Mark Schnitzer
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    A set of isobaric labeling reagents called TMTpro enables deep quantitative comparisons of proteome measurements across 16 samples.

    • Jiaming Li
    • , Jonathan G. Van Vranken
    • , Laura Pontano Vaites
    • , Devin K. Schweppe
    • , Edward L. Huttlin
    • , Chris Etienne
    • , Premchendar Nandhikonda
    • , Rosa Viner
    • , Aaron M. Robitaille
    • , Andrew H. Thompson
    • , Karsten Kuhn
    • , Ian Pike
    • , Ryan D. Bomgarden
    • , John C. Rogers
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    3D ATAC-PALM integrates ATAC with super-resolution imaging for nanoscale views of the accessible genome. When combined with FISH, protein fluorescence and genetic perturbation, the method enables investigation of accessible chromatin in situ.

    • Liangqi Xie
    • , Peng Dong
    • , Xingqi Chen
    • , Tsung-Han S. Hsieh
    • , Sambashiva Banala
    • , Margherita De Marzio
    • , Brian P. English
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    • , Seol Kyoung Jung
    • , Kyong-Rim Kieffer-Kwon
    • , Wesley R. Legant
    • , Anders S. Hansen
    • , Anton Schulmann
    • , Rafael Casellas
    • , Bin Zhang
    • , Eric Betzig
    • , Luke D. Lavis
    • , Howard Y. Chang
    • , Robert Tjian
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    DNA-based FluoroCubes are ~6 nm small, monovalent probes that contain six organic dyes, which confer dramatic photostability relative to single dyes and offer more uniform signal compared to quantum dots for extended biological imaging.

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News & Comment

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Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

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