In situ structure determination at nanometer resolution using TYGRESS

Latest Research

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    An adaptive excitation source enables two- and three-photon imaging of the awake mouse brain with high spatial and temporal resolution at 30-fold-reduced laser power relative to conventional approaches.

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  • Perspective |

    This Perspective highlights open-source software for single-cell analysis released as part of the Bioconductor project, providing an overview for users and developers.

    • Robert A. Amezquita
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    •  & Stephanie C. Hicks
  • Analysis |

    The 2018 Human Protein Atlas Image Classification competition sought to improve automated classification of protein subcellular localizations from fluorescence images. The winning strategies involved innovative deep learning approaches for multi-label classification.

    • Wei Ouyang
    • , Casper F. Winsnes
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    • , Hao Xu
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  • Article |

    The unique advantages of single-particle cryo-electron microscopy and cryo-electron tomography are combined in a method called TYGRESS, here applied to determine the structure of the intact ciliary axoneme at a resolution of 12 Å.

    • Kangkang Song
    • , Zhiguo Shang
    • , Xiaofeng Fu
    • , Xiaochu Lou
    • , Nikolaus Grigorieff
    •  & Daniela Nicastro
  • Article |

    An approach combining cryo-electron microscopy and mass spectrometry analysis of protein complexes enriched directly from cells enables structure determination of unknown complexes at atomic resolution.

    • Chi-Min Ho
    • , Xiaorun Li
    • , Mason Lai
    • , Thomas C. Terwilliger
    • , Josh R. Beck
    • , James Wohlschlegel
    • , Daniel E. Goldberg
    • , Anthony W. P. Fitzpatrick
    •  & Z. Hong Zhou
  • Article |

    Single-cell isolation following time-lapse imaging (SIFT) enables high-throughput screening of complex and dynamic phenotypes from pooled bacterial libraries. SIFT was used to generate ultraprecise synthetic gene oscillators.

    • Scott Luro
    • , Laurent Potvin-Trottier
    • , Burak Okumus
    •  & Johan Paulsson

News & Comment

Collection

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

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