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ATP-independent contractile proteins from plants

A Corrigendum to this article was published on 01 April 2005

Abstract

Emerging technologies are creating increasing interest in smart materials that may serve as actuators in micro- and nanodevices1,2,3. Mechanically active polymers currently studied include a variety of materials4,5,6,7,8,9. ATP-driven motor proteins, the actuators of living cells10, possess promising characteristics11,12,13, but their dependence on strictly defined chemical environments can be disadvantagous14. Natural proteins that deform reversibly by entropic mechanisms might serve as models for artificial contractile polypeptides with useful functionality15, but they are rare16. Protein bodies from sieve elements of higher plants17,18,19 provide a novel example. sieve elements form microfluidics systems for pressure-driven transport of photo-assimilates throughout the plant20,21,22. Unique protein bodies in the sieve elements of legumes act as cellular stopcocks, by undergoing a Ca2+-dependent conformational switch in which they plug the sieve element23. In living cells, this reaction is probably controlled by Ca2+-transporters in the cell membrane23. Here we report the rapid, reversible, anisotropic and ATP-independent contractility in these protein bodies in vitro. Considering the unique biological function of the legume 'crystalloid' protein bodies and their contractile properties, we suggest to give them the distinctive name forisome ('gate-body'; from the Latin foris, the wing of a gate).

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Figure 1: Response to Ca2+ of forisomes isolated from Vicia faba sieve elements.
Figure 2: Demonstration of mechanical force exerted by forisomes.
Figure 3: Forisome response to increasing pH.
Figure 4: Forisome swelling and curvature induced by electrotitration.

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Acknowledgements

We thank Gerhard Thiel for discussion of practical problems related to the determination of divalent cation concentrations in the nM range, and Jürgen Bereiter-Hahn, Julian Hibberd and Gerhard Thiel for critical comments on an early version of the manuscript. This paper is dedicated to the memory of Stephan Ohl, who died unexpectedly in 2002. His contribution to putting the forisome project on a sound financial basis was crucial.

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Correspondence to Michael Knoblauch.

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Knoblauch, M., Noll, G., Müller, T. et al. ATP-independent contractile proteins from plants. Nature Mater 2, 600–603 (2003). https://doi.org/10.1038/nmat960

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