 | Figure 2
Nature Medicine
9, 781 - 788 (2003)
Published online: 5 May 2003; | doi:10.1038/nm877
Leukocytes mediate retinal vascular remodeling during development and vaso-obliteration in diseaseSusumu Ishida, Kenji Yamashiro, Tomohiko Usui, Yuichi Kaji, Yuichiro Ogura, Tetsuo Hida, Yoshihito Honda, Yoshihisa Oguchi
& Anthony P Adamis | | | | Figure 2. Suppression of leukocyte adhesion and vascular pruning with CD18 blockade.
(a,b) CD18 blockade in rats reduced the number of adherent leukocytes on P5 (a), whereas the control antibody had no effect (b). (c) Leukocyte adhesion was significantly inhibited in the rats treated with antibody to CD18 (n = 12, 59.9  15.6 leukocytes) compared with the control group (n = 10, 119.2 21.9 leukocytes; *, P < 0.01). (d,e) CD18 blockade led to the suppression of vascular pruning on P6 (d), whereas vascular pruning proceeded normally in rats receiving control antibody (e). (f) There was a significant difference in vascular density within five disc diameters of the optic disc between rats treated with antibody to CD18 (n = 10, 36.7 3.3%) and control rats (n = 8, 27.3 2.7%; *, P < 0.01). (g) There was a significant difference in the number of branch-surrounded spaces located within five disc diameters of the optic disc between rats treated with antibody to CD18 (n = 10, 55.6 3.1) and control rats (n = 8, 38.7 2.9; *, P < 0.01). (h,i) CD18-deficient mice (h) had delayed vascular pruning in the periphery at P9 compared with age-matched wild-type mice (i). (j) There was a significant difference in vascular density within three disc diameters from the periphery between CD18-deficient mice (n = 10, 40.9 4.5%) and wild-type mice (n = 10, 24.5 4.8%; *, P < 0.01). Ab, antibody; anti-CD18 Ab, antibody to CD18; CD18-KO, CD18-deficient.
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