 | Figure 1
Nature Medicine
9, 453 - 457 (2003)
Published online: 3 March 2003; | doi:10.1038/nm838
Adult mouse astrocytes degrade amyloid- in vitro and in situTony Wyss-Coray, John D. Loike, Thomas C. Brionne, Emily Lu, Roman Anankov, Fengrong Yan, Samuel C. Silverstein
& Jens Husemann | | | | Figure 1. Adult mouse astrocytes cease migration upon interaction with A 1−42 and adhere to A1−42-coated surfaces.
a, Migration of astrocytes, in response to LPS or MCP-1, across porous membranes coated with CIV alone or with CIV and A 1−42. Number of cells that migrated across CIV-coated membranes under unstimulated conditions (214  74 cells/mm2; n = 4) was designated as 100%. #, P = 0.05 compared with no chemoattractant; †, P = 0.01 compared with LPS and A1−42; *, P = 0.05 compared with MCP-1 and A1−42. b, Adhesion of astrocytes, suspended in buffer with ( ) or without ( ) Ca2+ and Mg2+, to multi-spot glass slides that were uncoated, coated with CIV or coated with CIV and A1−42 (2  g/spot). c, Adhesion of astrocytes, suspended in buffer lacking Ca2+ and Mg2+, to multi-spot glass slides coated with CIV and increasing amounts of A1−42. d, Binding of Cy3-labeled A1−42 (Cy3-A1−42) to astrocytes with or without addition of 100 or 500 g/ml fucoidan, polyinosinic acid (polyI), or antibody against SRBI (SRBI). Values are mean s.e.m.; n = 3−5 experiments. *, P = 0.05 compared with basal; **, P = 0.01 compared with basal.
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