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Article
Nature Medicine  9, 1306 - 1312 (2003)
Published online: 14 September 2003; | doi:10.1038/nm929

Identification of PDGFR as a receptor for AAV-5 transduction

Giovanni Di Pasquale1, Beverly L Davidson2, 3, 4, Colleen S Stein2, Inês Martins2, Dominic Scudiero5, Anne Monks5 & John A Chiorini1

1  Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA.

2  Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.

3  Department of Neurology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.

4  Department of Physiology & Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.

5  SAIC-Frederick, Frederick, Maryland 21702, USA.

Correspondence should be addressed to John A Chiorini Jchiorini@dir.nidcr.nih.gov
Understanding the process of vector transduction has important implications for the application and optimal use of a vector system for human gene therapy. Recent studies with vectors based on adeno-associated virus type 5 (AAV-5) have shown utility of this vector system in the lung, central nervous system, muscle and eye. To understand the natural tropism of this virus and to identify proteins necessary for AAV-5 transduction, we characterized 43 cell lines as permissive or nonpermissive for AAV-5 transduction and compared the gene expression profiles derived from cDNA microarray analyses of those cell lines. A statistically significant correlation was observed between expression of the platelet-derived growth factor receptor (PDGFR-alpha-polypeptide) and AAV-5 transduction. Subsequent experiments confirmed the role of PDGFR-alpha and PDGFR-beta as receptors for AAV-5. The tropism of AAV-5 in vivo also correlated with the expression pattern of PDGFR-alpha.

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REFERENCE
Adeno-associated Viruses
Nature Encyclopaedia of Life Sciences
 See all 2 matches for Reference

REVIEWS
Neurological diseases: Viral vectors for gene delivery to the nervous system
Nature Reviews Neuroscience Review (01 May 2003)

NEWS AND VIEWS
Research Notes
Nature Biotechnology News and Views (01 Oct 2003)

RESEARCH
PKA/PrKX activity is a modulator of AAV/adenovirus interaction
The EMBO Journal Article (01 Apr 2003)
Adeno-associated viral glutamate decarboxylase expression in the lateral nucleus of the rat hypothalamus reduces feeding behavior
Gene Therapy Research Article (01 May 2004)

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Nature Medicine
ISSN: 1078-8956
EISSN: 1546-170X
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