Nature Medicine
7, 612 - 618 (2001)
doi:10.1038/87945
TGF- 1 promotes microglial amyloid- clearance and reduces plaque burden in transgenic miceTony Wyss-Coray1, 2, Carol Lin1, Fengrong Yan1, Gui-Qiu Yu1, Michelle Rohde1, Lisa McConlogue4, Eliezer Masliah5
& Lennart Mucke1, 2, 31
Gladstone Institute of Neurological Disease, University of California, San Francisco, California, USA
2
Department of Neurology, University of California, San Francisco, California, USA
3
Neuroscience Program, University of California, San Francisco, California, USA
4
Elan Pharmaceuticals, South San Francisco, California, USA
5
Departments of Neurosciences and Pathology, University of California, San Diego, La Jolla, California, USA
Correspondence should be addressed to Tony Wyss-Coray twysscoray@gladstone.ucsf.eduAbnormal accumulation of the amyloid- peptide (A) in the brain appears crucial to pathogenesis in all forms of Alzheimer disease (AD), but the underlying mechanisms in the sporadic forms of AD remain unknown. Transforming growth factor 1 (TGF-1), a key regulator of the brain's responses to injury and inflammation, has been implicated in A deposition in vivo. Here we demonstrate that a modest increase in astroglial TGF-1 production in aged transgenic mice expressing the human -amyloid precursor protein (hAPP) results in a three-fold reduction in the number of parenchymal amyloid plaques, a 50% reduction in the overall A load in the hippocampus and neocortex, and a decrease in the number of dystrophic neurites. In mice expressing hAPP and TGF-1, A accumulated substantially in cerebral blood vessels, but not in parenchymal plaques. In human cases of AD, A immunoreactivity associated with parenchymal plaques was inversely correlated with A in blood vessels and cortical TGF-1 mRNA levels. The reduction of parenchymal plaques in hAPP/TGF-1 mice was associated with a strong activation of microglia and an increase in inflammatory mediators. Recombinant TGF-1 stimulated A clearance in microglial cell cultures. These results demonstrate that TGF-1 is an important modifier of amyloid deposition in vivo and indicate that TGF-1 might promote microglial processes that inhibit the accumulation of A in the brain parenchyma.
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