 | Figure 2
Nature Medicine
7, 497 - 501 (2001)
doi:10.1038/86581
Subtelomeric chromosome rearrangements are detected using an innovative 12-color FISH assay (M-TEL)Jill Brown, Kaan Saracoglu, Sabine Uhrig, Michael R. Speicher, Roland Eils
& Lyndal Kearney | | | | Figure 2. Color classification (goldFISH) analysis of the telomeric signals in metaphases from patient 3.
a, The M-TEL1 probes identified a deletion of 7q on one chromosome 7 homolog (arrow). b, The M-TEL2 probes identified an additional chromosome 2 signal (orange) on the derivative chromosome 7 (arrow). One further FISH experiment with 2p and 2q subtelomeric probes was sufficient to confirm an unbalanced translocation resulting in monosomy for 7q and trisomy for 2q in this patient. The respective pseudocolors are given on the left. In both a and b, there are some apparent misclassifications, for example 5p and q, 11p, 16q, 19q. However, in all cases the misclassification involved only one out of a possible two sister chromatids on one chromosome arm. Note also that the 9q and XqYq PACs were not included in this early version of the M-TEL assay, because of cross-hybridization with several other telomeres18. Instead, the first-generation cosmids were applied separately in a dual-color hybridization. However, the newer BAC probes for 9q and XqYq do not cross-hybridize and were applied in the more recent M-TEL 1 hybridizations (see Table 1 and Fig. 4).
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